What we have learned from urinary bladder cancer models

What we have learned from urinary bladder cancer models Carina Bernardo1,2, Céu Costa1,4, Carlos Palmeira1,3,4, Rosário Pinto-Leite1,5, Paula Oliveira6, Rui Freitas7, Francisco Amado2, Lúcio L. Santos1,4,8 1Experimental Pathology and Therapeutics Group-Research Center, Portuguese Oncology Institute-Porto (IPO-Porto), Rua Dr. António Bernardino de Almeida, 4200-072 Porto, Portugal. 2Mass Spectrometry Group, QOPNA, Department of Chemistry, University of Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal. 3Department of Immunology, Portuguese Oncology Institute-Porto (IPO-Porto), Rua Dr. António Bernardino de Almeida, 4200-072 Porto, Portugal. 4 School of Health Sciences, Fernando Pessoa University, 4249-004 Porto, Portugal. 5 Genetic Service, Cytogenetic Laboratory, Hospital Center of Trás-os-Montes e Alto Douro, 5000-508 Vila Real, Portugal. 6Department of Veterinary Sciences, CITAB, University of Trás-os-Montes e Alto Douro, 5001-801 Vila Real, Portugal. 7Department of Urology, Portuguese Oncology Institute-Porto (IPO-Porto), Rua Dr. António Bernardino de Almeida, 4200-072 Porto, Portugal. 8Department of Surgical Oncology, Portuguese Oncology Institute-Porto (IPO-Porto), Rua Dr. António Bernardino de Almeida, 4200-072 Porto, Portugal. Correspondence to: Dr. Lúcio L. Santos, Portuguese Oncology Institute-Porto (IPO-Porto), Rua Dr. António Bernardino de Almeida, 4200-072 Porto, Portugal. E-mail: [email protected] Urinary bladder cancer (UBC) is a heterogeneous disease with highly variable clinical outcomes and responses to chemotherapy. Despite some advances in the molecular understanding of UBC, this knowledge still has not been translated to the clinic in terms of improvements in the prognosis and treatment of patients. Suitable urinary bladder tumor models representative of the human disease in terms of histology and behavior are needed to study factors involved in tumor initiation, progression and metastasis. Further, accurate model systems would facilitate identification of new therapeutic targets and predictive markers that could lead to optimization of existing therapies and development of new ones. Many established cancer cell lines derived from human urinary bladder tumors representing different grades and stages have been used as experimental models for UBC study. These cell lines reflect some of the genetic and morphologic alterations observed in human urothelial carcinoma and serve as simplified models to study the behavior of cancer cells in vitro. However, their translational potential is limited due to the artificial conditions, in which the cells are maintained, grown and tested. Animal models offer a more complex and realistic model for the establishment, development, and progression of tumors as well as to evaluate new therapeutic approaches. Over the years, the authors' group has worked with several UBC cell lines, established and characterized chemically induced UBC models, and patient-derived xenografts models. In this study, the authors will provide a summary of the UBC models developed by their group, analyze their translational potential and weaknesses, and define areas that remain to be explored.